The Moto2 Practice session was red-flagged due to weather conditions, but during the dry track time, Joe Roberts set the fastest time.
Before the rain returned to the Automotodrom Brno, it was Joe Roberts who jumped to the top of the timesheets for the OnlyFans American Racing Team, and that is where he remained when the red flag ended the session.
In second place was home hero, Filip Salac, the Czech rider was 0.542 seconds behind Roberts on his Elf Marc VDS Racing Team Boscoscuro.
The Liqui Moly Dynavolt Intact GP squad ended the day in third and fifth place, with Manuel Gonzalez in third and Senna Agius rounding out the top five.
It was Marcos Ramirez who split the Liqui Moly Dynavolt Intact GP teammates as he ended the session in fourth, but was 0.687 seconds behind his own teammate at the top of the order.
Mattia Pasini is participating in his first wildcard of the 2025 Moto2 World Championship, and the Italian ended the day in sixth place on the Fantic Racing Lino Sonego machine.
Behind Pasini was his teammate for the weekend, Aron Canet. The Spaniard, who is currently second in the Moto2 Championship, ended the day in seventh place.
British rider, Jake Dixon, secured a Q2 spot for Saturday as he ended up in eighth place on the timesheets for the Elf Marc VDS Racing Team.
Completing the top ten was the SpeedRS Team. Alonso Lopez ended the day in ninth place, and Celestino Vietti found himself in tenth.
Moto2 Czech Republic, Full Practice Results:
| 2025 Czech Republic Moto2 | Automotodrom Brno | Practice Results | Round 12 of 22 | |||||
| Pos | Name | Nat. | Team | Bike | Times |
| 1 | Joe Roberts | USA | OnlyFans American Racing Team | Kalex | 1:59.032 |
| 2 | Filip Salac | CZE | Elf Marc VDS Racing Team | Boscoscuro | 1:59.574 |
| 3 | Manuel Gonzalez | ESP | Liqui Moly Dynavolt Intact GP | Kalex | 1:59.604 |
| 4 | Marcos Ramirez | ESP | OnlyFans American Racing Team | Kalex | 1:59.719 |
| 5 | Senna Agius | AUS | Liqui Moly Dynavolt Intact GP | Kalex | 1:59.771 |
| 6 | Mattia Pasini | ITA | Fantic Racing Lino Sonego | Kalex | 1:59.906 |
| 7 | Aron Canet | ESP | Fantic Racing Lino Sonego | Kalex | 1:59.907 |
| 8 | Jake Dixon | GBR | Elf Marc VDS Racing Team | Boscoscuro | 1:59.991 |
| 9 | Alonso Lopez | ESP | SpeedRS Team | Boscoscuro | 2:00.022 |
| 10 | Celestino Vietti | ITA | SpeedRS Team | Boscoscuro | 2:00.090 |
| 11 | Zonta van den Goorbergh | NED | RW - Idrofoglia Racing | Kalex | 2:00.140 |
| 12 | Jorge Navarro | ESP | KLINT Forward Factory Team | Forward | 2:00.154 |
| 13 | Izan Guevara | ESP | BLU CRU Pramac Yamaha Moto2 | Boscoscuro | 2:00.167 |
| 14 | Collin Veijer | DEU | Red Bull KTM Ajo | Kalex | 2:00.220 |
| 15 | Deniz Oncu | TUR | Red Bull KTM Ajo | Kalex | 2:00.234 |
| 16 | Daniel Holgado | ESP | CFMOTO Gaviota Aspar Team | Kalex | 2:00.325 |
| 17 | Tony Arbolino | ITA | BLU CRU Pramac Yamaha Moto2 | Boscoscuro | 2:00.383 |
| 18 | David Alonso | COL | CFMOTO Gaviota Aspar Team | Kalex | 2:00.487 |
| 19 | Barry Baltus | BEL | Fantic Racing Lino Sonego | Kalex | 2:00.506 |
| 20 | Alex Escrig | ESP | KLINT Forward Factory Team | Forward | 2:00.667 |
| 21 | Ayumu Sasaki | JPN | RW - Idrofoglia Racing | Kalex | 2:00.678 |
| 22 | Albert Arenas | ESP | ITALJET Gresini Moto2 | Kalex | 2:00.752 |
| 23 | Darryn Binder | RSA | ITALJET Gresini Moto2 | Kalex | 2:01.012 |
| 24 | Adrian Huertas | ESP | Italtrans Racing Team | Kalex | 2:01.058 |
| 25 | Ivan Ortola | ESP | QJMOTOR - Frinsa - MSi | Boscoscuro | 2:01.060 |
| 26 | Yuki Kunii | JPN | IDEMITSU Honda Team Asia | Kalex | 2:01.314 |
| 27 | Taiga Hada | JPN | IDEMITSU Honda Team Asia | Kalex | 2:02.255 |
| 28 | Diogo Moreira | BRA | Italtrans Racing Team | Kalex | 2:02.448 |
| 29 | Eric Fernandez | ESP | QJMOTOR - Frinsa - MSi | Boscoscuro | 2:02.848 |
| 30 | Milan Pawelec | POL | AGR Team Fusport | Kalex | 2:03.130 |
