In MotoGP's first session back at the Automotodrom Brno, it was Marc Marquez who topped Free Practice One.
Marc Marquez topped the timesheets in the opening MotoGP session of the weekend at Automotodrom Brno, as he led the way by 0.615 seconds after missing the opening laps due to a technical issue.
As usual, Jack Miller found himself at the sharp end in the mixed conditions as he ended up in second place on the Prima Pramac Racing Yamaha.
Francesco Bagnaia ended the morning inside the top three on the factory Ducati, but he was 0.653 seconds behind his teammate.
Alex Marquez was worried for the Czech Republic Grand Prix, but in the latter laps he launched himself up the timesheets into fourth place.
After missing the German Grand Prix due to appendicitis, Enea Bastianini put himself in the top five in Free Practice One on the Red Bull KTM Tech3 RC16.
Pedro Acosta ended the session in sixth place on the Red Bull KTM Factory Racing machine, but he was 1.073 seconds behind Marquez at the end of the morning.
The top Aprilia rider in Free Practice One was Marco Bezzecchi on the factory Aprilia, as the Italian rider ended up in seventh place.
Luca Marini was the top Honda rider as he ended up in eighth place on the factory Honda HRC Castrol machine.
Alex Rins was the second Yamaha rider inside the top ten. Rumours suggest he is fighting for his future, so this weekend will be crucial for the Spaniard.
Completing the top ten was the LCR Honda IDEMITSU replacement rider, Takaaki Nakagami.
MotoGP Czech Republic, Full Free Practice One Results:
| 2025 Czech Republic MotoGP | Automotodrom Brno | Free Practice One Results | Round 12 of 22 | |||||
| Pos | Name | Nat. | Team | Bike | Gap |
| 1 | Marc Marquez | ESP | Ducati Lenovo Team | Ducati GP25 | 1:54.606 |
| 2 | Jack Miller | AUS | Prima Pramac Racing | Yamaha M1 | 1:55.221 |
| 3 | Pecco Bagnaia | ITA | Ducati Lenovo Team | Ducati GP25 | 1:55.259 |
| 4 | Alex Marquez | ESP | BK8 Gresini Racing MotoGP | Ducati GP24 | 1:55.371 |
| 5 | Enea Bastianini | ITA | Red Bull KTM Tech3 | KTM RC16 | 1:55.598 |
| 6 | Pedro Acosta | ESP | Red Bull KTM Factory Racing | KTM RC16 | 1:55.679 |
| 7 | Marco Bezzecchi | ITA | Aprilia Racing | Aprilia RS-GP | 1:55.864 |
| 8 | Luca Marini | ITA | Honda HRC Castrol | Honda RC213V | 1:55.945 |
| 9 | Alex Rins | ESP | Monster Energy Yamaha | Yamaha M1 | 1:56.169 |
| 10 | Takaaki Nakagami | JPN | LCR Honda IDEMITSU | Honda RC213V | 1:56.187 |
| 11 | Fabio Quartararo | FRA | Monster Energy Yamaha | Yamaha M1 | 1:56.259 |
| 12 | Miguel Oliveira | POR | Prima Pramac Racing | Yamaha M1 | 1:56.272 |
| 13 | Augusto Fernandez | ESP | Monster Energy Yamaha | Yamaha M1 | 1:56.531 |
| 14 | Johann Zarco | FRA | LCR Honda Castrol | Honda RC213V | 1:56.541 |
| 15 | Pol Espargaro | ESP | Red Bull KTM Tech3 | KTM RC16 | 1:56.617 |
| 16 | Fabio di Giannantonio | ITA | Pertamina Enduro VR46 Racing Team | Ducati GP25 | 1:57.073 |
| 17 | Jorge Martin | ESP | Aprilia Racing | Aprilia RS-GP | 1:57.128 |
| 18 | Raul Fernandez | ESP | Trackhouse Racing | Aprilia RS-GP | 1:57.431 |
| 19 | Joan Mir | ESP | Honda HRC Castrol | Honda RC213V | 1:57.715 |
| 20 | Fermin Aldeguer | ESP | BK8 Gresini Racing MotoGP | Ducati GP24 | 1:57.921 |
| 21 | Brad Binder | RSA | Red Bull KTM Factory Racing | KTM RC16 | 1:58.830 |
| 22 | Ai Ogura | JPN | Trackhouse Racing | Aprilia RS-GP | 2:11.904 |
