Adverse weather conditions made for a difficult Friday afternoon Practice session, but Marc Marquez found his way to the top once again.
It was business as usual for Marc Marquez as he dominated the opening day of Practice at the Automotodrom Brno, as he topped Free Practice One and Practice on Friday.
Johann Zarco was Marquez's closest challenger as he ended the day in second, but was 0.469 seconds behind the factory Ducati rider.
Fabio Quartararo and Monster Energy Yamaha gained direct access to Q2 as they ended up in third place on the timesheets.
Both Aprilia Racing riders ended the day inside the top five. Marco Bezzecchi was in fourth place, and the reigning MotoGP World Champion, Jorge Martin, was in fifth on his first day back in the paddock.
Jack Miller and difficult weather conditions tend to go hand in hand, and the Australian used them to his advantage on Friday as he ended up in sixth place.
Joan Mir was the second Honda rider to gain direct access to Q2 on Friday afternoon as he ended up in seventh place on the Honda HRC Castrol machine.
On his return to MotoGP after missing the German Grand Prix, Enea Bastianini was the top KTM rider in eighth place.
Despite a late crash, Alex Marquez was able to get out on his second bike and set a lap time that put him in ninth place.
Completing the top ten was Pedro Acosta on the Red Bull KTM Factory Racing machine.
MotoGP Czech Republic, Full Practice Results:
| 2025 Czech Republic MotoGP | Automotodrom Brno | Free Practice One Results | Round 12 of 22 | |||||
| Pos | Name | Nat. | Team | Bike | Times |
| 1 | Marc Marquez | ESP | Ducati Lenovo Team | Ducati GP25 | 2:03.935 |
| 2 | Johann Zarco | FRA | LCR Honda Castrol | Honda RC213V | 2:04.404 |
| 3 | Fabio Quartararo | FRA | Monster Energy Yamaha | Yamaha M1 | 2:04.465 |
| 4 | Marco Bezzecchi | ITA | Aprilia Racing | Aprilia RS-GP | 2:04.492 |
| 5 | Jorge Martin | ESP | Aprilia Racing | Aprilia RS-GP | 2:04.656 |
| 6 | Jack Miller | AUS | Prima Pramac Racing | Yamaha M1 | 2:04.716 |
| 7 | Joan Mir | ESP | Honda HRC Castrol | Honda RC213V | 2:04.811 |
| 8 | Enea Bastianini | ITA | Red Bull KTM Tech3 | KTM RC16 | 2:04.878 |
| 9 | Alex Marquez | ESP | BK8 Gresini Racing MotoGP | Ducati GP24 | 2:04.993 |
| 10 | Pedro Acosta | ESP | Red Bull KTM Factory Racing | KTM RC16 | 2:05.218 |
| 11 | Raul Fernandez | ESP | Trackhouse Racing | Aprilia RS-GP | 2:05.257 |
| 12 | Fermin Aldeguer | ESP | BK8 Gresini Racing MotoGP | Ducati GP24 | 2:05.305 |
| 13 | Pecco Bagnaia | ITA | Ducati Lenovo Team | Ducati GP25 | 2:05.440 |
| 14 | Alex Rins | ESP | Monster Energy Yamaha | Yamaha M1 | 2:05.473 |
| 15 | Brad Binder | RSA | Red Bull KTM Factory Racing | KTM RC16 | 2:05.500 |
| 16 | Fabio di Giannantonio | ITA | Pertamina Enduro VR46 Racing Team | Ducati GP25 | 2:05.627 |
| 17 | Pol Espargaro | ESP | Red Bull KTM Tech3 | KTM RC16 | 2:05.752 |
| 18 | Augusto Fernandez | ESP | Monster Energy Yamaha | Yamaha M1 | 2:05.799 |
| 19 | Miguel Oliveira | POR | Prima Pramac Racing | Yamaha M1 | 2:05.837 |
| 20 | Ai Ogura | JPN | Trackhouse Racing | Aprilia RS-GP | 2:06.308 |
| 21 | Takaaki Nakagami | JPN | LCR Honda IDEMITSU | Honda RC213V | 2:06.482 |
| 22 | Luca Marini | ITA | Honda HRC Castrol | Honda RC213V | 2:06.805 |
