A new all-time lap record allowed Marc Marquez to claim his 100th Grand Prix pole position across all classes.
Marc Marquez headed a Ducati front row lockout as he claimed pole position with a new all-time lap record at Mugello.
Francesco Bagnaia will start from second place on the grid alongside his factory Ducati teammate. The Italian could not match his teammate and will have to battle hard in the races.
Alex Marquez completed the Ducati front row lockout as he will start from third on the BK8 Gresini Racing MotoGP machine.
Despite all of his injury issues on Friday, Fabio Quartararo will start from the head of the second row in fourth place on the Monster Energy Yamaha.
Maverick Vinales was the top KTM rider in Qualifying as he put his Red Bull KTM Tech3 machine in fifth place on the grid in the middle of the second row.
Completing the second row of the grid was Franco Morbidelli on the Pertamina Enduro VR46 Racing Team Ducati GP24.
Fabio di Gianantonio was directly behind his teammate on the timesheets, but will start from the head of the third row on the grid in seventh place.
The second KTM rider in the top ten in MotoGP Qualifying was Pedro Acosta, as he qualified in eighth place on the Red Bull KTM Factory Racing RC16.
Alex Rins completed the third row of the grid as he qualified in ninth place on the Monster Energy Yamaha M1.
Completing the top ten was the top Aprilia rider as Marco Bezzecchi qualified at the head of the fourth row of the grid in tenth place.
MotoGP Italy, Full Qualifying Results:
| 2025 Italian MotoGP | Mugello Circuit | Qualifying Results | Round 9 of 22 | |||||
| Pos | Name | Nat. | Team | Bike | Times |
| 1 | Marc Marquez | ESP | Ducati Lenovo Team | Ducati GP25 | 1:44.169 |
| 2 | Pecco Bagnaia | ITA | Ducati Lenovo Team | Ducati GP25 | 1:44.228 |
| 3 | Alex Marquez | ESP | BK8 Gresini Racing MotoGP | Ducati GP24 | 1:44.252 |
| 4 | Fabio Quartararo | FRA | Monster Energy Yamaha | Yamaha M1 | 1:44.411 |
| 5 | Maverick Vinales | ESP | Red Bull KTM Tech3 | KTM RC16 | 1:44.514 |
| 6 | Franco Morbidelli | ITA | Pertamina Enduro VR46 Racing Team | Ducati GP24 | 1:44.568 |
| 7 | Fabio di Giannantonio | ITA | Pertamina Enduro VR46 Racing Team | Ducati GP25 | 1:44.723 |
| 8 | Pedro Acosta | ESP | Red Bull KTM Factory Racing | KTM RC16 | 1:44.780 |
| 9 | Alex Rins | ESP | Monster Energy Yamaha | Yamaha M1 | 1:44.844 |
| 10 | Marco Bezzecchi | ITA | Aprilia Racing | Aprilia RS-GP | 1:44.899 |
| 11 | Raul Fernandez | ESP | Trackhouse Racing | Aprilia RS-GP | 1:44.995 |
| 12 | Fermin Aldeguer | ESP | BK8 Gresini Racing MotoGP | Ducati GP24 | 1:45.199 |
| 13 | Jack Miller | AUS | Prima Pramac Racing | Yamaha M1 | Q1 |
| 14 | Johann Zarco | FRA | LCR Honda Castrol | Honda RC213V | Q1 |
| 15 | Brad Binder | RSA | Red Bull KTM Factory Racing | KTM RC16 | Q1 |
| 16 | Enea Bastianini | ITA | Red Bull KTM Tech3 | KTM RC16 | Q1 |
| 17 | Miguel Oliveira | POR | Prima Pramac Racing | Yamaha M1 | Q1 |
| 18 | Joan Mir | ESP | Honda HRC Castrol | Honda RC213V | Q1 |
| 19 | Takaaki Nakagami | JPN | Honda HRC Castrol | Honda RC213V | Q1 |
| 20 | Lorenzo Savadori | ITA | Aprilia Racing | Aprilia RS-GP | Q1 |
| 21 | Ai Ogura | JPN | Trackhouse Racing | Aprilia RS-GP | Q1 |
| 22 | Somkiat Chantra | THA | LCR Honda IDEMITSU | Honda RC213V | Q1 |
